Erachet: I wanna derive equations
Bad4: Good. So, if i give you the following equation: A(t) = (εdCd + εs1Cs + εs2Cs)b Where A(t) stands for UV absorbance with respect to t (temperature) and epsilon-d stands for the extinction coefficienct of the duplex strands. Cd is unknown Epsilon-s stands for the extinction coefficient of the single MO strand Cs is unknown Epsilon-s2 stands for th extinction coefficient of single DNA strand Cs again is unknown b = 1 and I give you all the epsilons for the given temperature of, say, 35 degrees celsius can you find Cs and Cd for me?
Erachet: er maybe?
Bad4: It's theoretically simple. Define the two variable in relation to each other and plug in. I think.
what are the epsilons?
Bad4: the funny e-shaped things.
Erachet: no, I know THAT
you said you would give me them
Bad4: They stand for extinction coefficient
oh, right. hold on: I'm going to email it to you. I wrote it all down, but I discovered that two of the epsilons are inaccurate. Theoretically, in terms of deriving the equations, it doesn't matter. But I'd like to get them right before I proceed because I'm a bit OCD like that.
Erachet: okay :)
...I'll get back to you after I eat some pizza bagels